Quantitative Analysis of CRISPR-Cas9 Efficiency in Targeted Gene Disruption Across Multiple Bacterial Species
CRISPR-Cas9 genome editing has revolutionized genetic engineering, yet its efficiency varies across bacterial species. This study evaluates the efficiency of CRISPR-Cas9 in disrupting a conserved housekeeping gene across six phylogenetically diverse bacterial species. We hypothesize that editing efficiency is influenced by species-specific factors, necessitating tailored optimization strategies. A comparative experimental design was used to disrupt a conserved gene (e.g., lacZ or rpsL) in Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Clostridium acetobutylicum, Lactobacillus pla
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